Peripherin antibody (N-Term)

Catalog No. ABIN229095

The Rabbit Polyclonal anti-Peripherin antibody (ABIN229095) specifically detects Peripherin in WB, IHC, IHC (p) and IHC (fro). The antibody is reactive with Human, Rat, Mouse, Cow and Pig samples.

Quick Overview for Peripherin antibody (N-Term) (ABIN229095)

Target: Peripherin (PRPH)

Reactivity: Human, Rat, Mouse, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Peripherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Peripherin Antibody

Binding Specificity: N-Term

Specificity: Peripherin. Stains a ~57kD band cleanly and specifically and does not stain vimentin, GFAP, alpha-internexin or any of the neurofilament subunits. Strong staining on rat, mouse, human, porcine and bovine peripherin. Does not stain chicken, quail or other more distantly related species which appear to lack peripherin.

Purification: Ion exchange chromatography

Immunogen: Electrophoretically pure trp-E-peripherin fusion protein (Dev. Brain Res. (1990) 57:235-248), containing all but the 4 N terminal amino acids of rat peripherin. Fusion protein purified from bacterial inclusion bodies by DEAE-cellulose chromatography in 6 M urea followed by preparative SDS-PAGE.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-Fr, IHC-P, WB

Usage: Suitable for use in Immunohistochemistry, Western Blot and Electron Microscopy. Immunohistochemistry: 1:100-1:200. Frozen sections fixed in acetone at -20?C. Works on tissue fixed for one hour or less in fresh 4 % paraformaldehyde. It has been reported that this antibody can be used on paraffin embedded tissue sections.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Neat serum. No preservative added.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Peripherin

Target: Peripherin (PRPH)

Alternative Name: Peripherin

Background: Name/Gene ID: PRPH

Synonyms: PRPH, NEF4, Neurofilament 4 (57kD), Peripherin, PRPH1, Neurofilament 4

Gene ID: 5630

UniProt: P41219