Crk antibody (pTyr221)

Catalog No. ABIN229989

The Rabbit Polyclonal anti-Crk antibody (ABIN229989) specifically detects Crk in WB and ELISA. The antibody is reactive with Human and Hamster samples.

Quick Overview for Crk antibody (pTyr221) (ABIN229989)

Target: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Reactivity: Human, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Crk antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Crk Antibody

Binding Specificity: pTyr221

Specificity: Detects endogenous levels of CrkII only when phosphorylated at tyrosine 221 at 42kD. Cross-reactivity: Tyr207 phosphorylated CrkL. Does not cross-react with other tyrosine-phosphorylated proteins. Species cross-reactivity: Human and hamster. Species sequence homology: mouse and rat 100 % .

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr221 of human CrkII.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, WB (1:1000)

Usage: Suitable for use in ELISA and Western Blot. Western Blot: 1:1000. Note: for Western Blots incubate membrane with diluted antibody in 5 % BSA, 1X TBS, and 0.1 % Tween-20 at 4?C with gentle shaking overnight.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.

Target Details for Crk

Target: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Alternative Name: CRK

Background: Name/Gene ID: CRK

Synonyms: CRK, Adapter molecule crk, CRKII, Proto-oncogene c-Crk, p38

Gene ID: 1398

UniProt: P46108

Pathways: Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor