MVP antibody

Catalog No. ABIN233326

This Mouse Monoclonal antibody specifically detects MVP in WB, IHC, FACS, IHC (p) and IP. It exhibits reactivity toward Human and Rat.

Quick Overview for MVP antibody (ABIN233326)

Target: MVP (Major Vault Protein (MVP))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MVP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-MVP Antibody

Specificity: Recognizes human major vault protein (MVP) at 104-110kD. Species cross-reactivity: rat.

Purification: Protein G purified

Immunogen: Proteins purified from extract of human breast cancer MCF-7 cells. Cellular Localization: Cytoplasmic.

Type of Immunogen: Cells

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: Flo, IHC, IHC-P, IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Native verified): 2 μg/mg protein lysate Use Protein G. Western Blot: 1-2 μg/mL for 2 hrs at RT. Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 20 minutes at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Positive control: SKBR3 cells and breast carcinomas.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MVP

Target: MVP (Major Vault Protein (MVP))

Alternative Name: MVP / LRP

Background: Name/Gene ID: MVP

Synonyms: MVP, Major vault protein, VAULT1, LRP

Gene ID: 9961

UniProt: Q14764