CHRM3 antibody (AA 580-589)

Catalog No. ABIN233474

The Rabbit Polyclonal anti-CHRM3 antibody is suitable to detect CHRM3 in samples from Human and Rat. It has been validated for ELISA, WB and IHC.

Quick Overview for CHRM3 antibody (AA 580-589) (ABIN233474)

Target: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRM3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CHRM3 Antibody

Binding Specificity: AA 580-589

Specificity: Recognizes rat M3 Muscarinic Receptor (aa580-589): 100 % , M3 Muscarinic Receptor 70 %, M1 Muscarinic Receptor (aa451-460) 0 %, M1 Muscarinic Receptor 0 %, M2 Muscarinic Receptor (aa457-466) 0 %, M2 Muscarinic Receptor 0 %, M4 Muscarinic Receptor (aa469-478) 0 %, M4 Muscarinic Receptor 0 %, M5 Muscarinic Receptor (aa419-531) 0 %, M5 Muscarinic Receptor 0 %. This antibody is also specific for the COOH terminal of the M3 receptor as characterized by Western Blot, ELISA and IHC.

Purification: Purified

Immunogen: A peptide analogue of the carboxyl terminal of the M3 receptor covalently attached to a carrier protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, WB

Usage: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Immunohistochemistry: 10 μg/mL had been found to stain rat brain sections that express the muscarinic M3 receptor after fixing with PLP fixative and using ABC techniques. Western Blot: homogenates whole rat brain.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Sterile PBS containing 10 mg/mL BSA

Concentration: Lot specific

Buffer: PBS, 10 mg/mL BSA.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CHRM3

Target: CHRM3 (Cholinergic Receptor, Muscarinic 3 (CHRM3))

Alternative Name: CHRM3 / M3

Background: Name/Gene ID: CHRM3
Subfamily: Acetylcholine
Family: GPCR

Synonyms: CHRM3, M3, Muscarinic receptor M3, Muscarinic M3 receptor, EGBRS, HM3, M3 muscarinic receptor

Gene ID: 1131

UniProt: P20309

Pathways: Synaptic Membrane