RAC1 antibody (pSer71)

Catalog No. ABIN233699

The Rabbit Polyclonal anti-RAC1 antibody has been validated for WB. It is suitable to detect RAC1 in samples from Human and Mouse.

Quick Overview for RAC1 antibody (pSer71) (ABIN233699)

Target: RAC1

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAC1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RAC1 Antibody

Binding Specificity: pSer71

Specificity: Detects endogenous Rac1 or cdc42 only when phosphorylated at Ser71. May also recognize phospho-RhoA (Ser73). Species cross-reactivity: Predicted to cross-react with mouse based on sequence homology.

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser71 of human Rac1 and cdc42.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (1:1000)

Usage: Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RAC1

Target: RAC1

Background: Name/Gene ID: RAC1
Family: Ras GTPase superfamily IPR001806

Synonyms: RAC1, MIG5, p21-Rac1, Ras-like protein TC25, TC25, TC-25, Rac-1

Gene ID: 5879

UniProt: P63000

Pathways: WNT Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling