TAF1 antibody (C-Term)

Catalog No. ABIN233826

Supplier Product No.: 600-401-995

Product Details anti-TAF1 Antibody

Target: TAF1 (TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TAF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Supplier Product No.: 600-401-995

Supplier: Rockland

Purpose: TAF1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human TAF1 protein.

Characteristics: Synonyms: Rabbit anti-TAF1 antibody, rabbit anti-Anti-Transcription Initiation Factor TFIID Subunit 1 antibody, TAF-1, TAF 1, Cell cycle gene 1 protein, TBP-associated factor 250 kDa, TAFII-250

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human TAF1.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 10 μg/mL

Application Note: This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, ChIP, and western blotting. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 213 kDa in size corresponding to TAF1 protein by western blotting in the appropriate cell lysate or extract.

ChIP Dilution: User Optimized

Western Blot Dilution: 1:100 - 1:2,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:150,000 - 1:300,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.15 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-TAF1 antibody (ABIN233826)

Gegonne, Tai, Zhang, Wu, Zhu, Yoshimoto, Hanson, Cultraro, Chen, Guinter, Yang, Hathcock, Singer, Rodriguez-Canales, Tessarollo, Mackem, Meerzaman, Buetow, Singer: "The general transcription factor TAF7 is essential for embryonic development but not essential for the survival or differentiation of mature T cells." in: Molecular and cellular biology, Vol. 32, Issue 10, pp. 1984-97, (2012) (PubMed).

Target Details for TAF1

Target: TAF1 (TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 250kDa (TAF1))

Alternative Name: TAF1 (TAF1 Products)

Background: Background: Anti-TAF1 antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Transcription Initiation Factor TFIID Subunit 1 (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex, which also includes TATA-binding protein (TBP) and a variety of TBP-associated factors.  TFIID nucleates the formation of transcription pre-initiation complexes and plays a key role in the regulation of gene expression by RNA polymerase II.  TAF1 possesses DNA-binding activity and also contains novel N- and C-terminal Ser/Thr kinase domains which can auto-phosphorylate or trans-phosphorylate other transcription factors. For example, TAF1 interacts with the C-terminus of TP53 and phosphorylates the T55 residue, leading to MDM2-mediated degradation of TP53.  TAF1 also catalyzes Ser phosphorylation of general transcription factor IIA (GTF2A1) and IIF (GTF2F1).  The retinoblastoma tumor suppressor protein, RB1, interacts with the N-terminal domain of TAF1 and inhibits its intrinsic kinase activity.  TAF1 is essential for progression through the G1 phase of the cell cycle and has been reported to be indispensable for expression of 18 % of mammalian genes.

Gene ID: 6872

NCBI Accession: NP_001273003

UniProt: P21675