WNT1 antibody (Internal Region)

Catalog No. ABIN233837

Supplier Product No.: 600-401-a37

There is 1 publication for this product available. The Rabbit Polyclonal anti-WNT1 antibody is suitable to detect WNT1 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for WNT1 antibody (Internal Region) (ABIN233837)

Target: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-WNT1 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-a37

Supplier: Rockland

Purpose: Wnt1 Antibody

Cross-Reactivity (Details): This antibody reacts with human and mouse Wnt1 protein.

Characteristics: Synonyms: rabbit anti-WNT-1 antibody, rabbit anti-WNT1 antibody, INT1 antibody, Murine mammary tumor virus integration site 1 antibody, Oncogene INT1 antibody, Proto oncogene protein Wnt 1 antibody, Wingless type MMTV integration site family member 1 antibody

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Wnt1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.

Western Blot Dilution: 1:1,500 - 1:6,000

ELISA Dilution: 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.38 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-WNT1 antibody (ABIN233837)

Banon-Maneus, Rovira, Ramirez-Bajo, Moya-Rull, Hierro-Garcia, Takenaka, Diekmann, Eickelberg, Königshoff, Campistol: "Wnt pathway activation in long term remnant rat model." in: BioMed research international, Vol. 2014, pp. 324713, (2015) (PubMed).

Target Details for WNT1

Target: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Alternative Name: WNT1

Background: Background: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt1 (Wingless-type MMTV integration site family member 1) is a member of the WNT gene family. It is highly conserved in evolution and the protein encoded by this gene is known to be 98 % identical to mouse Wnt1 protein at the amino acid level. Studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. Wnt1 is secreted as an extracellular matrix protein.

Gene ID: 7471, 4885655

UniProt: P04628

Pathways: WNT Signaling, Dopaminergic Neurogenesis