THRA antibody (N-Term)

Catalog No. ABIN233838

Supplier Product No.: 600-401-a38

Product Details anti-THRA Antibody

Target: THRA (Thyroid Hormone Receptor, alpha (THRA))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This THRA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-a38

Supplier: Rockland

Purpose: THRA Antibody

Cross-Reactivity (Details): This antibody reacts with human THRA protein.

Characteristics: Synonyms: rabbit anti-THRA antibody, rabbit anti-Thyroid hormone receptor alpha antibody, Nuclear receptor subfamily 1 group A member 1, c-erbA-alpha, c-erbA-1, V-erbA-related protein 7, EAR7, ERBA1, NR1A1, THRA1, THRA2

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human THRA isoform 1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:300,000

IF Microscopy Dilution: 1:200

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.89 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-THRA antibody (ABIN233838)

Contreras-Jurado, García-Serrano, Martínez-Fernández, Ruiz-Llorente, Paramio, Aranda: "Impaired hair growth and wound healing in mice lacking thyroid hormone receptors." in: PLoS ONE, Vol. 9, Issue 9, pp. e108137, (2015) (PubMed).

Mishra, Zhu, Ge, Cheng: "Adipogenesis is differentially impaired by thyroid hormone receptor mutant isoforms." in: Journal of molecular endocrinology, Vol. 44, Issue 4, pp. 247-55, (2010) (PubMed).

Target Details for THRA

Target: THRA (Thyroid Hormone Receptor, alpha (THRA))

Alternative Name: THRA (THRA Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Thyroid hormone receptor alpha is a nuclear hormone receptor with high affinity for the hormone triiodo-thyronine.  THRA is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having a certain extent of redundancy, may mediate different functions of thyroid hormone.  THRA interacts with NCOA3 and NCOA6 co-activators, leading to a strong increase in transcription of target genes. THRA is localized within the nucleus and has been found to exist as 4 isoforms originating from alternative splicing variants.  This antibody recognizes THRA isoform 1.  Isoform 1 has a distinct C-terminus compared to isoform 2.

Gene ID: 7067, 40806160

UniProt: P10827-2

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Maintenance of Protein Location, Skeletal Muscle Fiber Development