Shiga Toxin 1 & 2 (Stx-1, Stx-2) antibody

Catalog No. ABIN235035

This anti- antibody is a Mouse Monoclonal antibody detecting in ELISA, WB and Neut. Suitable for E. coli.

Quick Overview for Shiga Toxin 1 & 2 (Stx-1, Stx-2) antibody (ABIN235035)

Target: Shiga Toxin 1 & 2 (Stx-1, Stx-2)

Reactivity: E. coli

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Neutralization (Neut)

Clone: Mixed

Product Details

Specificity: Reacts with Escherichia coli (E. coli) Shigatoxin (STX) 1 & 2

Characteristics: MAb to E. coli STX 1 & 2, Monoclonal antibody to Escherichia coli (E. coli) Shigatoxin (STX) 1 & 2, pooled IgG

Purification: Protein A Chromatography. Ascites

Isotype: IgG

Application Details

Application Notes: Suitable for use in ELISA (capture antibody at 20- g/mL), Western blot (1- g/mL) and Toxin Neutralization. Neutralizes Vero Cell toxicity of E. coli O157:H7 extracts when used at a 5 µg/mL concentration. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Comment:

Concentration (detection method): 1 mg/mL (prior to lyophilization)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 1 mL deionized water.

Buffer: Lyophilized from 5 mM Phosphate buffer, pH 7.2 - 7.4.

Preservative: Without preservative

Handling Advice: Avoid repeated freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized product at 2-8 °C. After reconstitution, short term (up to 2 weeks) store at 2-8 °C. Long term (up to one year) aliquot and store at -28 °C. If aliquoted for long term storage, fill volume should be equal to or greater than 50 % of the nominal fill volume of the vial used.

Expiry Date: 12 months

Target Details

Target: Shiga Toxin 1 & 2 (Stx-1, Stx-2)