EGFR antibody (pSer1047)

Catalog No. ABIN235074

This anti-EGFR antibody is a Mouse Monoclonal antibody detecting EGFR in WB, ELISA and IFA. Suitable for Human.

Quick Overview for EGFR antibody (pSer1047) (ABIN235074)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Indirect Immunofluorescence Assay (IFA)

Clone: 1H9

Product Details anti-EGFR Antibody

Binding Specificity: pSer1047

Cross-Reactivity (Details): The EGF Receptor (EFR) is activated upon stimulation with growth factors of the EGF family and autophosphorylates at multiple tyrosine residues. Serine phosphorylation of the carboxy terminal domain at Serine 1047 leads to ligand-dependent EGFR desensitization. However, if serine 1047 is phosphorylated, the neighboring tyrosine 1045 may not be phosphorylated due to sterical hindrance, thus inhibiting cbl-mediated receptor degradation. Clone 1H9 specifically recognizes EGFR phosphorylated at Serine 1047. Reacts with Human, mouse and dog.

Characteristics: Cell lysate of 2 x 10^6 vanadate treated HepG2 cells.

Purification: Thiophilic adsorption and size exclusion Chromatography. Cell culture

Immunogen: Synthetic phosphopeptide corresponding to AA residues surrounding Serine 1047

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: 0.5 µg/mL for HRP/ECL detection. Recommended blocking buffer CPPT: 0.5 % (w/v) casein, 5 % (w/v) PEG 4000, 5 % (w/v) Polyvinylpyrrolidone (PVP), 0.5 % Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium Chloride. EGFR Transactivation Serum starved HepG2 cells were treated for 15 minutes as indicated. Whole cell lysates were separated by SDS-PAGE (20.000 cell per lane). The blot was probed with 0.5- g/mL K67190M for 1 hour at RT and developed by ECL (exp. time: 30 sec). Lane 1: control Lane 5: Sorbit Lane 2: PMA Lane 6: Anisomycin Lane 3: Forskolin Lane 7: Lonomycin Lane 4: LPA Lane 8: Taxol

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 1 mL water for 15 minutes at room temperature.

Buffer: Lyophilized from PBS, PEG, and Sucrose, 0.09 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Store lyophilized product at -20 °C. After reconstitution, aliquot and store at -80 °C. Thawed aliquots may be stored at 2-0 °C for up to 3 months.

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins