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Goat anti-Human IgG (Fc Region) Antibody (HRP)

Polyclonal HRP F(ab')2 fragment
Catalog No. ABIN237483
  • Target See all IgG products
    IgG
    Fragment
    F(ab')2 fragment
    Binding Specificity
    • 2398
    • 515
    • 417
    • 146
    • 129
    • 128
    • 67
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 1
    • 1
    Fc Region
    Reactivity
    • 687
    • 677
    • 573
    • 433
    • 381
    • 208
    • 195
    • 144
    • 127
    • 126
    • 107
    • 102
    • 96
    • 88
    • 82
    • 58
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 1706
    • 1431
    • 525
    • 205
    • 112
    • 111
    • 32
    • 31
    • 21
    • 18
    • 15
    • 11
    • 11
    Goat
    Clonality
    • 3131
    • 172
    • 16
    • 1
    Polyclonal
    Conjugate
    • 587
    • 416
    • 415
    • 367
    • 274
    • 262
    • 235
    • 118
    • 85
    • 72
    • 67
    • 64
    • 61
    • 58
    • 58
    • 44
    • 39
    • 39
    • 37
    • 35
    • 35
    • 32
    • 31
    • 31
    • 30
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 23
    • 21
    • 21
    • 19
    • 17
    • 17
    • 16
    • 15
    • 15
    • 15
    • 15
    • 13
    • 10
    • 8
    HRP
    Cross-Reactivity (Details)
    Based on immunoelectrophoresis, the antibody reacts with the heavy chains on human IgG but not with the light chains on most human immunoglobulins. No antibody was detected against human IgM, IgA, or against non-immunoglobulin serum proteins. Has been passed through solid phase immunoadsorbent gels to remove antibodies which cross-react with bovine, horse, and mouse serum proteins, but antibodies may cross-react with immunoglobulins from other species. Minimal cross-reaction to Bovine, Horse, and Mouse Serum Proteins.
    Characteristics
    Goat anti Human IgG (Fc) F(ab' )2 Fragment, Goat antibody to Human Immunoglobulin G (IgG), Fc Fragment Specific Horseradish Peroxidase conjugated
    Purification
    Isolated from antisera by a combination of pepsin digestion and Immuno Affinity Chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
  • Comment

    Concentration (detection method): 0.8 mg/mL (prior to lyophilization)

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute to 0.5 mL with distilled water.
    Buffer
    Lyophilized from 0.01 M Sodium Phosphate, 0.21 M Sodium Chloride, pH 7.6 containing 15 mg/mL BSA.
    Handling Advice
    Avoid repeated freezing and thawing. Dilute only prior to immediate use
    Centrifuge product if not completely clear after standing at room temperature.
    Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
    Storage
    4 °C
    Storage Comment
    Store lyophilized product at 2-8 °C. After reconstitution, this product is stable for several weeks at 2-8 °C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50 % glycerol and store at -28 °C, with or without aliquoting.
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
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