Mycobacterium Tuberculosis Antigen CFP10 (Rv3874) antibody

Catalog No. ABIN238347

The Rabbit Polyclonal anti-Mycobacterium Tuberculosis Antigen CFP10 (Rv3874) antibody (ABIN238347) specifically detects Mycobacterium Tuberculosis Antigen CFP10 (Rv3874) in WB. The antibody is reactive with Mycobacterium tuberculosis samples.

Quick Overview for Mycobacterium Tuberculosis Antigen CFP10 (Rv3874) antibody (ABIN238347)

Target: Mycobacterium Tuberculosis Antigen CFP10 (Rv3874)

Reactivity: Mycobacterium tuberculosis

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Specificity: The polyclonal antibody reacts with CFP10 (LHP), a 12,3 kDa antigen encoded by gene Rv3874 of Mycobacterium tuberculosis. The antibody recognizes CFP10 of Mycobacterium tuberculosis H37Rv and Mycobacterium bovis, it reacts also with recombinant antigen produced in Escherichia coli.

Purification: Protein-A affinity chromatography.

Immunogen: CFP10 (Rv3874) of Mycobacterium tuberculosis

Application Details

Application Notes: Suitable for Western Blotting under Reducing conditions: 0.2-1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: Mycobacterium Tuberculosis Antigen CFP10 (Rv3874)

Alternative Name: Cfp10 / EsxB

Target Type: Bacteria

Background: CFP10 (10 kDa culture filtrate protein) is a low molecular weight protein isolated from Mycobacterium tuberculosis culture filtrates. CFP10 and the 6 kDa early secretory antigen of T cells (ESAT6) are secreted in abundance by Mycobacterium tuberculosis and are frequently recognized by T cells from infected people. The genes encoding these proteins have been deleted from the genome of the vaccine strain Bacille Calmette Guerin (BCG). CFP10 is one of a small number of secreted M. tuberculosis proteins now identified, that appear to play key roles in tuberculosis pathogenesis and in the stimulation of immunity.Synonyms: 10 kDa culture filtrate antigen cfp10, ESAT-6-like protein esxB, MT3988, MTV027.09, Rv3874, Secreted antigenic protein MTSA-10, lhp, mtsA10

Gene ID: 886194

NCBI Accession: NP_218391

UniProt: P0A566