CD73 antibody (AA 27-252)

Catalog No. ABIN238367

The Mouse Monoclonal anti-CD73 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect CD73 in samples from Human.

Quick Overview for CD73 antibody (AA 27-252) (ABIN238367)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD73 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 2B6

Product Details anti-CD73 Antibody

Binding Specificity: AA 27-252

Specificity: This antibody is specific for NT5E/CD73.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: NT5, NTE, NT5E, 5'-nucleotidase

Purification: Protein-G affinity chromatography

Immunogen: Recombinant Human CD73 (aa 27-252) purified from E. coli

Isotype: IgG2b

Application Details

Application Notes: ELISA. Western blot: 1/1,000 - 1/2,000. Immunohistochemistry on Paraffin Sections: 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 with 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: CD73

Background: CD73 (Ecto-5'-nucleotidase, 5'-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.Synonyms: 5'-nucleotidase, NT5, NT5E, NTE

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process