RNLS antibody (Internal Region)

Catalog No. ABIN238548

The Goat Polyclonal anti-RNLS antibody (ABIN238548) specifically detects RNLS in WB and ELISA. The antibody is reactive with Mouse and Rat samples.

Quick Overview for RNLS antibody (Internal Region) (ABIN238548)

Target: RNLS (Renalase, FAD-Dependent Amine Oxidase (RNLS))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This RNLS antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-RNLS Antibody

Binding Specificity: Internal Region

Purpose: Renalase (aa 134 to 147)

Sequence: QINLRDDKWE VSKQ

Specificity: This antibody is expected to recognize both reported isoforms (NP_001026879.1 and NP_060833.1).

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QINLRDDKWEVSKQ, from the Internal region of the protein sequence according to NP_001026879.1, NP_060833.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 37 kDa band observed in Mouse Kidney and Rat Kidney lysates (calculated MW of 37.8 kDa according to Human NP_001026879.1 and 35.0 kDa according to Rat NP_001014189.1). Recommended concentration: 0.5-1.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for RNLS

Target: RNLS (Renalase, FAD-Dependent Amine Oxidase (RNLS))

Alternative Name: C10orf59

Background: Renalase, C10orf59, chromosome 10 open reading frame 59, FLJ11218

Gene ID: 55328

NCBI Accession: NP_001026879, NP_060833