IGFBP3 antibody (Internal Region)

Catalog No. ABIN238551

The Goat Polyclonal anti-IGFBP3 antibody (ABIN238551) specifically detects IGFBP3 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for IGFBP3 antibody (Internal Region) (ABIN238551)

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IGFBP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-IGFBP3 Antibody

Binding Specificity: Internal Region

Purpose: IGFBP3

Sequence: RYKVDYESQS TDTQN

Specificity: This antibody is expected to recognise both reported isoforms (NP_001013416.1 and NP_000589.2).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RYKVDYESQSTDTQN, from the internal region of the protein sequence according to NP_001013416.1, NP_000589.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 28 kDa band observed in Human Breast Cancer lysates (calculated MW of 31.7 kDa according to NP_001013416.1 and 32.2 kDa according to NP_000589.2). Recommended concentration: 0.02-0.06 μg/mL. An additional band of 50 kDa was consistently

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IGFBP3

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Alternative Name: IGFBP3

Background: IGFBP3, insulin-like growth factor binding protein 3, tcag7.703, BP-53, IBP3, IGF-binding protein 3, acid stable subunit of the 140 K IGF complex , binding protein 29, binding protein 53, growth hormone-dependent binding protein

Gene ID: 3486, 16009, 24484

NCBI Accession: NP_001013416, NP_000589

Pathways: Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding