ADRB3 antibody (N-Term)

Catalog No. ABIN238553

The Goat Polyclonal anti-ADRB3 antibody has been validated for WB and ELISA. It is suitable to detect ADRB3 in samples from Human.

Quick Overview for ADRB3 antibody (N-Term) (ABIN238553)

Target: ADRB3 (Adrenergic, beta-3-, Receptor (ADRB3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ADRB3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-ADRB3 Antibody

Binding Specificity: N-Term

Purpose: ADRB3

Sequence: PHENSSLAPW PD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence PHENSSLAPWPD-C, from the N Terminus of the protein sequence according to NP_000016.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 45 kDa band observed in Human Brain (Cortex) lysates (calculated MW of 43.5 kDa according to NP_000016.1). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ADRB3

Target: ADRB3 (Adrenergic, beta-3-, Receptor (ADRB3))

Alternative Name: ADRB3

Background: ADRB3, adrenergic, beta-3-, receptor, BETA3AR, Beta-3 Adrenergic Receptor

Gene ID: 155

NCBI Accession: NP_000016

Pathways: cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Brown Fat Cell Differentiation