HCRTR2 antibody (N-Term)

Catalog No. ABIN238589

The Goat Polyclonal anti-HCRTR2 antibody has been validated for ELISA and WB. It is suitable to detect HCRTR2 in samples from Human. There is 1 publication available.

Quick Overview for HCRTR2 antibody (N-Term) (ABIN238589)

Target: HCRTR2 (Hypocretin (Orexin) Receptor 2 (HCRTR2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HCRTR2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Grade: Verified

Product Details anti-HCRTR2 Antibody

Binding Specificity: N-Term

Purpose: Orexin Receptor 2

Sequence: SGTKLEDSPP CRN

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence SGTKLEDSPPCRN, from the N Terminus of the protein sequence according to NP_001517.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 50.7 kDa according to NP_001517.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-HCRTR2 antibody (ABIN238589)

Ji, Zhang, Chen, Wang, Zhu: "Orexin prevents depressive-like behavior by promoting stress resilience." in: Molecular psychiatry, Vol. 24, Issue 2, pp. 282-293, (2020) (PubMed).

Target Details for HCRTR2

Target: HCRTR2 (Hypocretin (Orexin) Receptor 2 (HCRTR2))

Alternative Name: HCRTR2

Background: OTTHUMP00000039969, hypocretin (orexin) receptor 2, HCRTR2, OX2R, hypocretin receptor-2, orexin receptor 2

Gene ID: 3062, 25605

NCBI Accession: NP_001517

Pathways: Feeding Behaviour