EPB41L2 antibody (Internal Region)

Catalog No. ABIN238594

This Goat Polyclonal antibody specifically detects EPB41L2 in ELISA, IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for EPB41L2 antibody (Internal Region) (ABIN238594)

Target: EPB41L2 (erythrocyte Membrane Protein Band 4.1-Like 2 (EPB41L2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EPB41L2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-EPB41L2 Antibody

Binding Specificity: Internal Region

Purpose: EPB41L2 / 4.1G (aa 593 to 604)

Sequence: RREVRSPTKA PH

Specificity: This antibody is reported to recognise all isoforms NP_001422.1 (iso a), NP_001129026.1 (iso b), NP_001186317.1 (iso c), NP_001239589.1 (iso d), NP_001337228.1 (iso e), NP_001337230.1 (iso f), NP_001337232.1 (iso g),NP_001337233.1 (iso h), NP_001337236.1

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RREVRSPTKAPH, from the internal region of the protein sequence according to NP_001422.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Uterus and Colon. Recommended concentration: 2.5 μg/mL.

Western Blot: Preliminary testing showed bands at approx 110+40 kDa in Human Cerebellum lysatesafter 0.1 μg/mL antibody staining (calculated MW of 113 kDa according to NP_001422.1 These bands were successfully blocked by incubation with the immunizing pept

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membranes of A431 and in the cell junctions of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Re

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EPB41L2

Target: EPB41L2 (erythrocyte Membrane Protein Band 4.1-Like 2 (EPB41L2))

Alternative Name: EPB41L2

Background: EPB41L2, 4.1-G, erythrocyte membrane protein band 4.1-like 2, RP3-324N14.1, DKFZp781D1972, DKFZp781H1755, OTTHUMP00000040262, erythrocyte membrane protein band 4.1 like-protein 2

Gene ID: 2037

NCBI Accession: NP_001422