Enkephalin antibody (Internal Region)

Catalog No. ABIN238618

This Goat Polyclonal antibody specifically detects Enkephalin in WB, IHC and ELISA. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for Enkephalin antibody (Internal Region) (ABIN238618)

Target: Enkephalin (PENK) (Proenkephalin (PENK))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Enkephalin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-Enkephalin Antibody

Binding Specificity: Internal Region

Purpose: Proenkephalin

Sequence: RSHHQDGSDN EE

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RSHHQDGSDNEE, from the internal region of the protein sequence according to NP_006202.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Frozen section of Human Hypothalamus shows staining of dense enkephalinergic axon plexus and scattered neuronal cell bodies (higher magnification inset) in the human infundibular nucleus. Recommended concentration: 0.3-1 μg/mL.

Western Blot: Approx 37 kDa band observed in Human Adrenal Gland lysates (calculated MW of 30.8 kDa according to NP_006202.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Normant and Loh, Endocrino

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Enkephalin antibody (ABIN238618)

Skrapits, Borsay, Herczeg, Ciofi, Liposits, Hrabovszky: "Neuropeptide co-expression in hypothalamic kisspeptin neurons of laboratory animals and the human." in: Frontiers in neuroscience, Vol. 9, pp. 29, (2015) (PubMed).

Target Details for Enkephalin

Target: Enkephalin (PENK) (Proenkephalin (PENK))

Alternative Name: PENK

Background: Proenkephalin, PENK

Gene ID: 5179

NCBI Accession: NP_006202

Pathways: Stem Cell Maintenance