EPB41L5 antibody (Internal Region)

Catalog No. ABIN238631

The Goat Polyclonal anti-EPB41L5 antibody has been validated for WB, ELISA, FACS and IF. It is suitable to detect EPB41L5 in samples from Human.

Quick Overview for EPB41L5 antibody (Internal Region) (ABIN238631)

Target: EPB41L5 (Erythrocyte Membrane Protein Band 4.1 Like 5 (EPB41L5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EPB41L5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-EPB41L5 Antibody

Binding Specificity: Internal Region

Purpose: EBPL41L5

Sequence: ENLPQSPGTD QHD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ENLPQSPGTDQHD, from the internal region of the protein sequence according to NP_065960.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 80 kDa band observed in lysates of cell line A431 and approx. 75 kDa in lysates of cell line MCF7 (calculated MW of 81.9 kDa according to NP_065960.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperatur

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in U2OS and A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EPB41L5

Target: EPB41L5 (Erythrocyte Membrane Protein Band 4.1 Like 5 (EPB41L5))

Alternative Name: EPB41L5

Background: EPB41L5, erythrocyte membrane protein band 4.1 like 5, BE37, FLJ12957, KIAA1548

Molecular Weight: 81.9kDa

Gene ID: 57669

NCBI Accession: NP_065960