PC antibody (Internal Region)

Catalog No. ABIN238663

This Goat Polyclonal antibody specifically detects PC in WB, IHC and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for PC antibody (Internal Region) (ABIN238663)

Target: PC (Pyruvate Carboxylase (PC))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-PC Antibody

Binding Specificity: Internal Region

Purpose: Pyruvate Carboxylase

Sequence: KFKEVKKAYV EANQ

Specificity: All reported variants (NP_000911.2, NP_001035806.1, NP_071504.2) represent identical protein.

Predicted Reactivity: Human, Mouse, Rat, Dog, Cow, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-KFKEVKKAYVEANQ

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Approx 150 kDa band observed in Mouse Liver lysates (calculated MW of 130 kDa according to NP_000911.2, NP_001035806.1 and NP_071504.2). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.

DS IHC Results: Paraffin embedded Human Liver. Recommended concentration: 5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PC

Target: PC (Pyruvate Carboxylase (PC))

Alternative Name: Pyruvate Carboxylase

Background: PC, pyruvate carboxylase, PCB

Gene ID: 5091

NCBI Accession: NP_000911, NP_001035806, NP_071504