D Amino Acid Oxidase antibody (C-Term)

Catalog No. ABIN238666

This Goat Polyclonal antibody specifically detects D Amino Acid Oxidase in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for D Amino Acid Oxidase antibody (C-Term) (ABIN238666)

Target: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This D Amino Acid Oxidase antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-D Amino Acid Oxidase Antibody

Binding Specificity: C-Term

Purpose: D-amino-acid oxidase

Sequence: EEKKLSRMPP SH

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EEKKLSRMPPSH, from the C Terminus of the protein sequence according to NP_001908.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Cerebellum shows pixulated cytoplasm staining in neurons directly near the granular layer. Recommended concentration: 2-4 μg/mL.

Western Blot: Preliminary experiments in human brain (cerebellum, hippocampus, substantia nigra) lysates gave no specific signal but low background (at antibody concentration up to 1 μg/mL).

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for D Amino Acid Oxidase

Target: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Alternative Name: DAO

Background: DAO, D-amino-acid oxidase, DAAO, DAMOX, MGC35381, OXDA

Gene ID: 1610

NCBI Accession: NP_001908