Aromatase antibody (Internal Region)

Catalog No. ABIN238672

Product Details anti-Aromatase Antibody

Target: Aromatase (CYP19A1) (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Aromatase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: Aromatase / CYP19A1

Sequence: HDLSLHPDET KN

Specificity: This antibody is expected to recognise both reported isoforms (NP_000094.2 and NP_112503.1), which are identical

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-HDLSLHPDETKN, from the internal region of the protein sequence according to NP_000094.2, NP_112503.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 4 μg/mL.

Western Blot: Approx 50-55 kDa band observed in Human Placenta lysates (calculated MW of 57.9 kDa according to NP_000094.2 and NP_112503.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Aromatase

Target: Aromatase (CYP19A1) (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1))

Alternative Name: CYP19A1 (CYP19A1 Products)

Background: CYP19A1, cytochrome P450, family 19, subfamily A, polypeptide 1 , ARO, ARO1, CPV1, CYAR, CYP19, MGC104309, P-450AROM, aromataseN: cytochrome P450, family 19, cytochrome P450, subfamily XIX (aromatization of androgens), estrogen synthetase, flavoprotein-li

Gene ID: 1588

NCBI Accession: NP_000094, NP_112503

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis