Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) (AA 485-503), (C-Term) antibody

Details for Product No. ABIN3044414
Target
Binding Specificity
AA 485-503, C-Term
16
12
10
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
92
64
39
6
4
4
4
4
3
2
2
2
2
2
2
1
1
1
Host
Rabbit
71
21
7
Clonality
Polyclonal
Conjugate
Un-conjugated
5
4
4
4
4
4
4
3
3
2
2
2
2
2
2
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
59
30
26
24
21
16
11
7
5
3
1
Options
Purpose Rabbit IgG polyclonal antibody for Aromatase(CYP19A1) detection. Tested with WB, IHC-P in Human.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Aromatase(485-503aa KNMLEMIFTPRNSDRCLEH).
Sequence KNMLEMIFTP RNSDRCLEH
Isotype IgG
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Aromatase(CYP19A1) detection. Tested with WB, IHC-P in Human.
Gene Name: cytochrome P450, family 19, subfamily A, polypeptide 1
Protein Name: Aromatase
Purification Immunogen affinity purified.
Target
Alternative Name CYP19A1 (CYP19A1 Antibody Abstract)
Background Aromatase is an enzyme responsible for a key step in the biosynthesis of estrogens. It is a member of the cytochrome P450 superfamily, which are monooxygenases that catalyze many reactions involved in steroidogenesis. In particular, aromatase is responsible for the aromatization of androgens into estrogens. The CYP19 gene spans at least 70 kb of genomic DNA and contains 10 exons. By in situ hybridization, the ARO gene is mapped to 15q21.1. The aromatase enzyme can be found in many tissues including gonads, brain, adipose tissue, placenta, blood vessels, skin, bone, and endometrium, as well as in tissue of endometriosis, uterine fibroids, breast cancer, and endometrial cancer. It is an important factor in sexual development. Some bodybuilders taking steroids also take antiaromatase supplements to prevent excess testosterone conversion into estrogens, which can cause gynecomastia.

Synonyms: ARO 1 antibody|ARO antibody|ARO1 antibody|Aromatase antibody|CP19A_HUMAN antibody|CPV antibody|CPV1 antibody|CYAR antibody|CYP19 antibody|CYP19A1 antibody|CYPXIX antibody|Cytochrome P-450AROM antibody|Cytochrome P450 19A1 antibody|Cytochrome P450 family 19 subfamily A polypeptide 1 antibody|Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody|Cytochrome P450, subfamily XIX(aromatization of androgens) antibody|Estrogen synthase antibody|Estrogen synthetase antibody|Flavoprotein linked monooxygenase antibody|MGC104309 antibody|Microsomal monooxygenase antibody|OTTHUMP00000162543 antibody|OTTHUMP00000198350 antibody|P 450AROM antibody|P450AROM antibody
UniProt P11511
Pathways Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months
Images
Image no. 1 for anti-Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) (AA 485-503), (C-Term) antibody (ABIN3044414) Anti-Aromatase antibody, IHC(P) IHC(P): Human Placenta Tissue
Image no. 2 for anti-Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1) (AA 485-503), (C-Term) antibody (ABIN3044414) Anti-Aromatase antibody, Western blotting Lane 1: Human Placenta Tissue Lysate Lane 2...
Product cited in: Zhang, Zhang, Zhang, Lu, Li, Cui: "MiRNA-143 mediates the proliferative signaling pathway of FSH and regulates estradiol production." in: The Journal of endocrinology, Vol. 234, Issue 1, pp. 1-14, 2017 (PubMed).

Zhang, Yang, Zou, Lu, Xiong, Wu, Wang, Gao, Xu, Zou: "Estradiol synthesis and release in cultured female rat bone marrow stem cells." in: BioMed research international, Vol. 2013, pp. 301540, 2013 (PubMed).

Zhao, Tian, Hao, Chen: "Extragonadal aromatization increases with time after ovariectomy in rats." in: Reproductive biology and endocrinology : RB&E, Vol. 3, pp. 6, 2006 (PubMed).

Zhao, Tian, Chen: "Electroacupuncture stimulates hypothalamic aromatization." in: Brain research, Vol. 1037, Issue 1-2, pp. 164-70, 2005 (PubMed).