Myogenin antibody (AA 30-224)

Catalog No. ABIN241574

The Mouse Monoclonal anti-Myogenin antibody is suitable to detect Myogenin in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IHC (p), ICC and IHC (fro).

Quick Overview for Myogenin antibody (AA 30-224) (ABIN241574)

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Myogenin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 5FD

Product Details anti-Myogenin Antibody

Binding Specificity: AA 30-224

Specificity: The 5FD clone has been highly published, users are encouraged to consult the scientific literature for additional application information. In Rh-30, a 34 kD band should be observed. The can be used for gel shift assays.

Purification: Protein G purified

Immunogen: A recombinant GST fusion protein corresponding to amino acids 30-224 of rat myogenin.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ICC (5 μg/mL), IHC, IHC-Fr (5 - 10 μg/mL), IHC-P (10 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Short term: 4°C
Long term: -70°C.

Target Details for Myogenin

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Alternative Name: MYOG / Myogenin

Background: Name/Gene ID: MYOG

Synonyms: MYOG, BHLHc3, Myogenin, MYF4, Myogenic factor 4, Myf-4, Myogenin (myogenic factor 4)

Gene ID: 4656

UniProt: P15173

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development