anti-HRP antibody

Catalog No. ABIN2443813

This Mouse Monoclonal antibody specifically detects HRP in IHC. It exhibits reactivity toward Horseradish.

Quick Overview for anti-HRP antibody (ABIN2443813)

Target: HRP (Horseradish Peroxidase (HRP))

Reactivity: Horseradish

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (IHC)

Clone: MAP A6-2

Product Details anti-HRP Antibody

Purpose: Horseradish Peroxidase (HRP) Monoclonal Antibody (MAP A6-2)

Specificity: A sensitive antibody against horseradish peroxidase (HRP). This antibody was raised against the full horseradish peroxidase (HRP) protein

Purification: Protein G purified

Immunogen: HRP protein

Isotype: IgG1

Application Details

Application Notes: IHC 1:30

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: IHC 1:30 - 1:60. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for HRP

Background: Horseradish peroxidase (HRP) is a 40 kDa glycoprotein with four lysine residues. In the presence of hydrogen peroxide, HRP will oxidize a substrate to yield a detectable signal useful for techniques such as ELISA and immunohistochemistry. A monoclonal mouse anti-peroxidase antibody is a very useful and reliable reagent for the localization of a number of mouse primary antibodies by peroxidase anti-peroxidase (PAP) immunocytochemistry.