Nucleobindin (full length) antibody

Catalog No. ABIN2443849

This Rabbit Polyclonal antibody specifically detects Nucleobindin in ELISA, ICC, IF, IP and WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for Nucleobindin (full length) antibody (ABIN2443849)

Target: Nucleobindin

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Product Details

Binding Specificity: full length

Purpose: Nucleobindin Rabbit Polyclonal Antibody

Specificity: The antibody recognizes nucleobindin 1 (NucB1)

Purification: Immunogen affinity purified

Immunogen: Full length recombinant human nucleobindin protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μg: WB: 1:1000, IF: 1:150, EM-ICC: 1:50. Optimal dilution has to be determined by the user.

Buffer: Lyophilized immunogen affinity purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details

Target: Nucleobindin

Background:

NUCB1, NUC, CALNUC

Nucleobindin 1 is a 461 amino acids ubiquitous protein with an N-terminal of 25 amino acids signal peptide responsible for its initial localization to the endoplasmic reticulum (ER). It has also been detected in the cytoplasm and in various subcellular structures such as the nucleus, mitochondria and the Golgi apparatus. Nucleobindin plays a role in a number of cellullar processes such as intracellular signaling, inflammation, autoimmunity, osteogenesis and cancer.

UniProt: Q02818