GST-Tag antibody

Catalog No. ABIN2443852

This Mouse Monoclonal antibody specifically detects GST-Tag in WB, ELISA, IP, DB and IHC. It exhibits reactivity toward Schistosoma japonicum.

Quick Overview for GST-Tag antibody (ABIN2443852)

Target: GST-Tag

Reactivity: Schistosoma japonicum

Host: Mouse

Clonality: Monoclonal

Conjugate: This GST-Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB), Immunohistochemistry (IHC)

Clone: GST-B6

Product Details anti-GST-Tag Antibody

Purpose: GST Tag Mouse Monoclonal Antibody (GST.B6)

Specificity: The antibody recognizes native and denatured forms of purified GST or GST fusion proteins.

Purification: Protein G purified

Immunogen: The antibody was raised against the GST protein.

Isotype: IgG2a

Application Details

Application Notes: WB 1:1000,IHC 1:500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:1000-3,000, IHC 1:500-2,000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for GST-Tag

Target: GST-Tag

Alternative Name: GST Tag

Target Type: Tag

Background: Glutathione-S-transferases (GSTs) are ubiquitous multifunctional enzymes, which play a key role in cellular detoxification. The GST gene is often used in gene fusion systems for the expression, purification and detection of fusion proteins.