PRNP antibody (pSer43)

Catalog No. ABIN2443909

This Rabbit Polyclonal antibody specifically detects PRNP in WB, ELISA, IHC and IP. It exhibits reactivity toward Mammalian.

Quick Overview for PRNP antibody (pSer43) (ABIN2443909)

Target: PRNP (Prion Protein (PRNP))

Reactivity: Mammalian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRNP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-PRNP Antibody

Binding Specificity: pSer43

Purpose: Phospho Prion Protein (pPrP Ser43) Rabbit Polyclonal Antibody

Sequence: PGGNRYPC

Specificity: A highly specific and sensitive antibody against the phosphorylation of the Ser43 of the Prion Protein. The antibody reacts with the Ser43 (G[pSer]PGGNRYPC) of the Phosphorylated Prion Protein.

Immunogen: Phospho-peptide

Application Details

Application Notes: WB 1:100,IP 1:100,IHC 1:200-1:1000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 50 μL: ELISA (phospho peptide) 1:500,000, E (non-phospho peptide) 1:25,000 WB 1:100, IP 1:100, IHC 1:200-1:1000. Optimal dilution has to be determined by the user

Buffer: Lyophilized serum

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for PRNP

Target: PRNP (Prion Protein (PRNP))

Alternative Name: Prion Protein

Background:

anti-Phospho Prion Protein, anti-Prion Protein, PrP, ASCR, PrP27-30, PrP33-35C , CD_antigen: CD230

Prion related neurodegenerative diseases, called transmissible spongiform encephalopathies, are observed in many animal species. These diseases involve conversion of normal cellular prion protein (PrPc) into a form that is insoluble and resistant to proteases (PrPSc). The protease resistant form can polymerize into fibrils which accumulate in infected tissues and cause cell death and tissue damage. PrPs have an N-terminal signal sequence and a C-terminal linkage to glycosylphosphatidylinositol anchor. The mature protein is a glycosylated protein that associates with cell membranes. Phosphorylation of PrPC at Ser-43 by Cdk5 promotes proteinase K resistance, prion aggregation, and fibril formation in vitro. In addition, Ser-43 phosphorylation is upregulated in scrapie-infected mouse brain relative to controls. Thus, phosphorylation of Ser-43 may be an important mechanism leading conversion of PrPc to PrPSc and the onset of disease. The antibody was raised against Phospho Prion Protein (pPrP Ser43).

UniProt: P04156

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation