Antigen O2 antibody

Catalog No. ABIN2444014

This Mouse Monoclonal antibody specifically detects Antigen O2 in Aggl and ELISA. It exhibits reactivity toward Pseudomonas aeruginosa.

Quick Overview for Antigen O2 antibody (ABIN2444014)

Target: Antigen O2

Reactivity: Pseudomonas aeruginosa

Host: Mouse

Clonality: Monoclonal

Application: Agglutination (Aggl), ELISA

Clone: MF71-2

Product Details

Purpose: Pseudomonas Aeruginosa antigen O2 Mouse Monoclonal Antibody

Specificity: The antibody recognizes the O2 serotype of Pseudomonas aeruginosa and it might cross-react with O5, O16, O18 and O20.

Purification: Purified

Immunogen: Whole bacteria strain O2

Isotype: IgM

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with deionized water. Agglutination (10 μg/assay). Optimal dilution has to be determined by the user.

Buffer: Lyophilized purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details

Target: Antigen O2

Background:

P. Aeruginosa antigen O2, serotype 2

Pseudomonas aeruginosa is a Gram-negative bacterium widely distributed in nature and causing opportunistic infections in humans. P. aeruginosa is an important bacterial pathogen of nosocomial (hospital derived) infections, and it can also cause life threatening diseases in patients with cancer, burn wounds, cystic fibrosis and those that have received immunosuppressive therapy. Classification of P. aeruginosa isolates is an important and routine task in hospitals. The basis for the serotyping system for P. aeruginosa is the differences among the O antigen side chains of lipopolysaccharide (LPS). A large number of O antigen side chains have been established by various investigators around the world which led to the creation of a standardized serotyping system known as the International Antigenic Typing Scheme (IATS) with twenty characterized O serotype strains of P. aeruginosa.