CBX5 antibody (AA 179-191)

Catalog No. ABIN2452033

This Rabbit Polyclonal antibody specifically detects CBX5 in WB and IF. It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for CBX5 antibody (AA 179-191) (ABIN2452033)

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBX5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-CBX5 Antibody

Binding Specificity: AA 179-191

Cross-Reactivity (Details): Not tested in other species.

Characteristics: The product is prepared by immunizing rabbit with the synthetic peptide CEDAENKEKETAKS corresponding to the amino acid sequence 179-191 of human HP1alpha and purified by affinity purification with the peptide. The antiserum was prepared by the direction of Prof. T. Haraguchi.

Purification: Affinity purified

Sterility: Sterile filtered

Isotype: IgG

Application Details

Application Notes: 1) Western blotting: 1/1,000~1/3,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.37 mg/mL

Buffer: 0.12 M sodium phosphate buffer ( pH 7.4), 50 % glycerol

Preservative: Azide free

Storage: -20 °C/-80 °C

Storage Comment: -20 C (For long term storage: -70 C)

References for anti-CBX5 antibody (ABIN2452033)

Lomberk, Wallrath, Urrutia: "The Heterochromatin Protein 1 family." in: Genome biology, Vol. 7, Issue 7, pp. 228, (2007) (PubMed).

Wang, Koyama, Nishida, Haraguchi, Reith, Tsukamoto: "The assembly and maintenance of heterochromatin initiated by transgene repeats are independent of the RNA interference pathway in mammalian cells." in: Molecular and cellular biology, Vol. 26, Issue 11, pp. 4028-40, (2006) (PubMed).

Kametaka, Takagi, Hayakawa, Haraguchi, Hiraoka, Yoneda: "Interaction of the chromatin compaction-inducing domain (LR domain) of Ki-67 antigen with HP1 proteins." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 7, Issue 12, pp. 1231-42, (2002) (PubMed).

Target Details for CBX5

Target: CBX5 (Chromobox Homolog 5 (CBX5))

Alternative Name: HP1 alpha/cbx5

Background: Heterochromatin protein 1 (HP1) is a major component of heterochromatin and plays a role in assembly of various proteins on chromatin and gene silencing. The HP1 family is evolutionally conserved, with members in fungi, plants and animals but not prokaryotes, and there are multiple members within the same species. The HP1 family proteins are encoded by a class of genes known as the chromobox (CBX) genes. In humans, HP1alpha is encoded by the Chromobox homolog 5 (CBX5) gene located on chromosome 12q13.13. HP1alpha has been observed to interact directly or indirectly with several non-histone proteins with a wide variety of functions.

UniProt: P45973