NUP98 antibody (AA 1-466)

Catalog No. ABIN2452066

This Rat Monoclonal antibody specifically detects NUP98 in WB, ELISA, IF, ICC and DB. It exhibits reactivity toward Human, Mouse, Rat, Saccharomyces cerevisiae and Schizosaccharomyces pombe and has been mentioned in 2+ publications.

Quick Overview for NUP98 antibody (AA 1-466) (ABIN2452066)

Target: NUP98 (Nucleoporin 98kDa (NUP98))

Reactivity: Human, Mouse, Rat, Saccharomyces cerevisiae, Schizosaccharomyces pombe

Host: Rat

Clonality: Monoclonal

Conjugate: This NUP98 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Dot Blot (DB)

Clone: 2H10

Product Details anti-NUP98 Antibody

Binding Specificity: AA 1-466

Cross-Reactivity (Details): This antibody does not react with Tetrahymena.
Other species have not been tested.

Purification: Purified

Sterility: Sterile filtered

Immunogen: Recombinant GST-fused human Nup98 (aa 1-466)

Isotype: IgG2c kappa

Application Details

Application Notes: 1) Western blotting
2) Immunocytochemistry
3) ELISA
4) Dot blotting

Properties:
When injected into the cytoplasm, this antibody accumulates into the nuclear pores of HeLa cells and inhibits nuclear localization of endogenous Ran.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol. Azide- and carrier protein-free.

Preservative: Azide free

Storage: -20 °C

Storage Comment: Upon arrival centrifuge briefly and store at -20 C.

References for anti-NUP98 antibody (ABIN2452066)

Iwamoto, Asakawa, Ohtsuki, Osakada, Koujin, Hiraoka, Haraguchi: "Monoclonal antibodies recognize gly-leu-phe-gly repeat of nucleoporin nup98 of tetrahymena, yeasts, and humans." in: Monoclonal antibodies in immunodiagnosis and immunotherapy, Vol. 32, Issue 2, pp. 81-90, (2013) (PubMed).

Fukuhara, Ozaki, Shikata, Katahira, Yoneda, Ogino, Tachibana: "Specific monoclonal antibody against the nuclear pore complex protein, nup98." in: Hybridoma (2005), Vol. 24, Issue 5, pp. 244-7, (2005) (PubMed).

Target Details for NUP98

Target: NUP98 (Nucleoporin 98kDa (NUP98))

Alternative Name: Nup98

Background: Nucleoporin 98 (Nup98) is a component of nuclear pore complex (NPC), which is a large protein assembly embedded in the nuclear envelope. It is localized on both nuclear and cytoplasmic side of NPC. This protein contains glycine-leucine-phenylalanine-glycine (GLFG) amino acid repeats and plays a critical role in nuclear trafficking. Nup98 plays a specific role in the RNA export. Nup98 gene is fused to a variety of partner genes in human myeloid and T-cell malignancies via chromosomal translocation. This hybridoma has been established by Prof. T. Tachibana's Lab. at Osaka City Univ.

UniProt: Q9HDC8

Pathways: Stem Cell Maintenance, Protein targeting to Nucleus, SARS-CoV-2 Protein Interactome