DHX32 antibody

Catalog No. ABIN2458244

The Rabbit Polyclonal anti-DHX32 antibody has been validated for WB and ELISA. It is suitable to detect DHX32 in samples from Human.

Quick Overview for DHX32 antibody (ABIN2458244)

Target: DHX32 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 32 (DHX32))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DHX32 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DHX32 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DHX32.

Application Details

Application Notes: DHX32 antibody can be used for detection of DHX32 by ELISA at 1:62500. DHX32 antibody can be used for detection of DHX32 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store DHX32 antibody at -20 °C.

Target Details for DHX32

Target: DHX32 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 32 (DHX32))

Alternative Name: DHX32

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DHX32 is a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants, but the full length nature of one of the variants has not been defined. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants, but the full length nature of one of the variants has not been defined.

Molecular Weight: 84 kDa

Gene ID: 55760

NCBI Accession: NP_060650

UniProt: Q7L7V1