SUPT16H antibody

Catalog No. ABIN2458347

This anti-SUPT16H antibody is a Rabbit Polyclonal antibody detecting SUPT16H in ELISA and WB. Suitable for Human, Mouse and Rat.

Quick Overview for SUPT16H antibody (ABIN2458347)

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUPT16H antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-SUPT16H Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SUPT16H.

Application Details

Application Notes: SUPT16H antibody can be used for detection of SUPT16H by ELISA at 1:1562500. SUPT16H antibody can be used for detection of SUPT16H by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store SUPT16H antibody at -20 °C.

Target Details for SUPT16H

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Alternative Name: SUPT16H

Background: Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, the latter of which is SUPT16H. Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, the latter of which is the protein encoded by this gene. Sequence Note: The sequence AF152961.1 is a chimeric mRNA clone. Only the SUPT16H region was propagated into this RefSeq record. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Molecular Weight: 120 kDa

Gene ID: 11198

NCBI Accession: NP_009123

UniProt: Q9Y5B9

Pathways: Chromatin Binding