Keratin 10 antibody

Catalog No. ABIN2458601

The Rabbit Polyclonal anti-Keratin 10 antibody has been validated for WB and ELISA. It is suitable to detect Keratin 10 in samples from Human, Mouse, Rat and Dog.

Quick Overview for Keratin 10 antibody (ABIN2458601)

Target: Keratin 10 (KRT10)

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Keratin 10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Keratin 10 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KRT10.

Application Details

Application Notes: KRT10 antibody can be used for detection of KRT10 by ELISA at 1:62500. KRT10 antibody can be used for detection of KRT10 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store KRT10 antibody at -20 °C.

Target Details for Keratin 10

Target: Keratin 10 (KRT10)

Alternative Name: KRT10

Background: KRT10 is a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in the gene encoding KRT10 are associated with epidermolytic hyperkeratosis. This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Molecular Weight: 59 kDa

Gene ID: 3858

NCBI Accession: NP_000412

UniProt: P13645