FBXO21 antibody

Catalog No. ABIN2458715

The Rabbit Polyclonal anti-FBXO21 antibody is suitable to detect FBXO21 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for FBXO21 antibody (ABIN2458715)

Target: FBXO21 (F-Box Protein 21 (FBXO21))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO21 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FBXO21 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FBXO21.

Application Details

Application Notes: FBXO21 antibody can be used for detection of FBXO21 by ELISA at 1:312500. FBXO21 antibody can be used for detection of FBXO21 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store FBXO21 antibody at -20 °C.

Target Details for FBXO21

Target: FBXO21 (F-Box Protein 21 (FBXO21))

Alternative Name: FBXO21

Background: FBXO21 contains 1 F-box domain. It is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants.

Molecular Weight: 71 kDa

Gene ID: 23014

NCBI Accession: NP_055817

UniProt: O94952