POMT1 antibody

Catalog No. ABIN2458911

The Rabbit Polyclonal anti-POMT1 antibody (ABIN2458911) specifically detects POMT1 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for POMT1 antibody (ABIN2458911)

Target: POMT1 (Protein-O-Mannosyltransferase 1 (POMT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POMT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-POMT1 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POMT1.

Application Details

Application Notes: POMT1 antibody can be used for detection of POMT1 by ELISA at 1:62500. POMT1 antibody can be used for detection of POMT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store POMT1 antibody at -20 °C.

Target Details for POMT1

Target: POMT1 (Protein-O-Mannosyltransferase 1 (POMT1))

Alternative Name: POMT1

Background: POMT1 is an O-mannosyltransferase that requires interaction with the product of the POMT2 gene for enzymatic function. The encoded protein is found in the membrane of the endoplasmic reticulum. Defects in this gene are a cause of Walker-Warburg syndrome (WWS) and limb-girdle muscular dystrophy type 2K (LGMD2K).O-mannosylation is an important protein modification in eukaryotes that is initiated by an evolutionarily conserved family of protein O-mannosyltransferases. POMT1 shares sequence similarity with protein O-mannosyltransferases of S. cerevisiae. In yeast, these enzymes are located in the endoplasmic reticulum (ER) and are required for cell integrity and cell wall rigidity. POMT1 also shows similarity to the Drosophila 'rotated abdomen' (rt) gene, which when mutated causes defects in myogenesis and muscle structure.

Molecular Weight: 82 kDa

Gene ID: 10585

NCBI Accession: NP_001070833

UniProt: B3KQG0