The Rabbit Polyclonal anti-CSNK2A1/CK II alpha antibody has been validated for WB and ELISA. It is suitable to detect CSNK2A1/CK II alpha in samples from Human, Mouse, Rat, Drosophila melanogaster and C. elegans.
CSNK2A1 antibody can be used for detection of CSNK2A1 by ELISA at 1:62500. CSNK2A1 antibody can be used for detection of CSNK2A1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice
As with any antibody avoid repeat freeze-thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
For short periods of storage (days) store at 4 °C. For longer periods of storage, store CSNK2A1 antibody at -20 °C.
Target
CSNK2A1/CK II alpha (CSNK2A1)
(Casein Kinase 2 alpha 1 (CSNK2A1))
Alternative Name
CSNK2A1
Background
Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. CSNK2A1 represents the alpha subunit.Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene.