POU3F2 antibody

Catalog No. ABIN2460197

The Rabbit Polyclonal anti-POU3F2 antibody has been validated for WB and ELISA. It is suitable to detect POU3F2 in samples from Human, Rat, Mouse and Dog.

Quick Overview for POU3F2 antibody (ABIN2460197)

Target: POU3F2 (POU Class 3 Homeobox 2 (POU3F2))

Reactivity: Human, Rat, Mouse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU3F2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-POU3F2 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POU3F2.

Application Details

Application Notes: POU3F2 antibody can be used for detection of POU3F2 by ELISA at 1:12500. POU3F2 antibody can be used for detection of POU3F2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store POU3F2 antibody at -20 °C.

Target Details for POU3F2

Target: POU3F2 (POU Class 3 Homeobox 2 (POU3F2))

Alternative Name: POU3F2

Background: N-Oct-3 (POU3F2) is a protein belonging to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, which occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1) and Oct2 (POU2F2), and the pituitary protein Pit1 (PIT1). Class III POU genes are expressed predominantly in the CNS. It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression.N-Oct-3 is a protein belonging to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, which occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1, MIM 164175) and Oct2 (POU2F2, MIM 164176), and the pituitary protein Pit1 (PIT1, MIM 173110). Class III POU genes are expressed predominantly in the CNS. It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression.[supplied by OMIM].

Molecular Weight: 47 kDa

Gene ID: 5454

NCBI Accession: NP_005595

UniProt: P20265