EN2 antibody

Catalog No. ABIN2460205

This Rabbit Polyclonal antibody specifically detects EN2 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for EN2 antibody (ABIN2460205)

Target: EN2 (Engrailed Homeobox 2 (EN2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EN2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-EN2 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EN2.

Application Details

Application Notes: EN2 antibody can be used for detection of EN2 by ELISA at 1:62500. EN2 antibody can be used for detection of EN2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store EN2 antibody at -20 °C.

Target Details for EN2

Target: EN2 (Engrailed Homeobox 2 (EN2))

Alternative Name: EN2

Background: Homeobox-containing genes are thought to have a role in controlling development. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Molecular Weight: 34 kDa

Gene ID: 2020

NCBI Accession: NP_001418

UniProt: P19622