YAF2 antibody

Catalog No. ABIN2460230

The Rabbit Polyclonal anti-YAF2 antibody is suitable to detect YAF2 in samples from Human and Dog. It has been validated for WB, IHC and ELISA.

Quick Overview for YAF2 antibody (ABIN2460230)

Target: YAF2 (YY1 Associated Factor 2 (YAF2))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YAF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-YAF2 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human YAF2.

Application Details

Application Notes: YAF2 antibody can be used for detection of YAF2 by ELISA at 1:62500. YAF2 antibody can be used for detection of YAF2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store YAF2 antibody at -20 °C.

Target Details for YAF2

Target: YAF2 (YY1 Associated Factor 2 (YAF2))

Alternative Name: YAF2

Background: YAF2 interacts with YY1, a zinc finger protein involved in negative regulation of muscle-restricted genes. YAF2 contains a single N-terminal C2-X10-C2 zinc finger, and in contrast to YY1, is up-regulated during myogenic differentiation. It also facilitates proteolytic cleavage of YY1 by the calcium- activated protease, m-calpain, suggesting a mechanism by which this protein antagonizes the negative effect of YY1.The protein encoded by this gene interacts with YY1, a zinc finger protein involved in negative regulation of muscle-restricted genes. This gene product itself contains a single N-terminal C2-X10-C2 zinc finger, and in contrast to YY1, is up-regulated during myogenic differentiation. It also facilitates proteolytic cleavage of YY1 by the calcium- activated protease, m-calpain, suggesting a mechanism by which this protein antagonizes the negative effect of YY1. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two have been confirmed to date.

Molecular Weight: 20 kDa

Gene ID: 10138

NCBI Accession: NP_005739

UniProt: Q8IY57