SUPT16H antibody

Catalog No. ABIN2460242

The Rabbit Polyclonal anti-SUPT16H antibody has been validated for ELISA and WB. It is suitable to detect SUPT16H in samples from Human and Dog.

Quick Overview for SUPT16H antibody (ABIN2460242)

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUPT16H antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-SUPT16H Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SUPT16H.

Application Details

Application Notes: SUPT16H antibody can be used for detection of SUPT16H by ELISA at 1:312500. SUPT16H antibody can be used for detection of SUPT16H by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store SUPT16H antibody at -20 °C.

Target Details for SUPT16H

Target: SUPT16H (FACT complex subunit SPT16 (SUPT16H))

Alternative Name: SUPT16H

Background: Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, the latter of which is SUPT16H.

Molecular Weight: 66 kDa, 120 kDa

Gene ID: 11198

UniProt: Q9Y5B9

Pathways: Chromatin Binding