GLI1 antibody

Catalog No. ABIN2460438

The Rabbit Polyclonal anti-GLI1 antibody (ABIN2460438) specifically detects GLI1 in WB and ELISA. The antibody is reactive with Human and Dog samples.

Quick Overview for GLI1 antibody (ABIN2460438)

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GLI1 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GLI1.

Application Details

Application Notes: GLI1 antibody can be used for detection of GLI1 by ELISA at 1:312500. GLI1 antibody can be used for detection of GLI1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store GLI1 antibody at -20 °C.

Target Details for GLI1

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Alternative Name: GLI1

Background: GLI1 is a protein which is a member of the Kruppel family of zinc finger proteins. The function of this gene has not been determined, however, it may play a role in normal development gene transcription. Mouse mutation studies indicate possible involvement in human foregut malformation.

Molecular Weight: 118 kDa

Gene ID: 2735

NCBI Accession: NP_005260

UniProt: P08151

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis