NFIX antibody

Catalog No. ABIN2460546

The Rabbit Polyclonal anti-NFIX antibody (ABIN2460546) specifically detects NFIX in WB and ELISA. The antibody is reactive with Human, Rat, Mouse and Dog samples.

Quick Overview for NFIX antibody (ABIN2460546)

Target: NFIX (MLZE) (Nuclear Factor I/X (MLZE))

Reactivity: Human, Rat, Mouse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFIX antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NFIX Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFIX.

Application Details

Application Notes: NFIX antibody can be used for detection of NFIX by ELISA at 1:312500. NFIX antibody can be used for detection of NFIX by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NFIX antibody at -20 °C.

Target Details for NFIX

Target: NFIX (MLZE) (Nuclear Factor I/X (MLZE))

Alternative Name: NFIX

Background: Nuclear factor I (NFI) proteins constitute a family of sequence-specific transcription factors whose functional diversity is generated through transcription from four different genes (NFI-A, NFI-B, NFI-C, and NFI-X), alternative RNA splicing, and protein heterodimerization. NFI-X has divergent functions after binding in promoter or enhancer position. This property, combined with the differential expression of NFI-X, can achieve cell-type specificity of NFI dependent promoters and enhancers.

Molecular Weight: 49 kDa

Gene ID: 4784

NCBI Accession: NP_002492

Pathways: Skeletal Muscle Fiber Development