Claudin 1 antibody

Catalog No. ABIN2460770

The Rabbit Polyclonal anti-Claudin 1 antibody has been validated for WB, IHC and ELISA. It is suitable to detect Claudin 1 in samples from Human and Dog.

Quick Overview for Claudin 1 antibody (ABIN2460770)

Target: Claudin 1 (CLDN1)

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claudin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Claudin 1 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CLDN1.

Application Details

Application Notes: CLDN1 antibody can be used for detection of CLDN1 by ELISA at 1:312500. CLDN1 antibody can be used for detection of CLDN1 by western blot at 1.0-5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store CLDN1 antibody at -20 °C.

Target Details for Claudin 1

Target: Claudin 1 (CLDN1)

Alternative Name: CLDN1

Background: Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. This protein, a member of the claudin family, is an integral membrane protein and a component of tight junction strands.

Molecular Weight: 23 kDa

Gene ID: 9076

NCBI Accession: NP_066924

UniProt: O95832

Pathways: Cell-Cell Junction Organization, Hepatitis C