Pirin antibody

Catalog No. ABIN2460778

This Rabbit Polyclonal antibody specifically detects Pirin in ELISA, WB and IHC. It exhibits reactivity toward Human, Mouse and Dog.

Quick Overview for Pirin antibody (ABIN2460778)

Target: Pirin (PIR)

Reactivity: Human, Mouse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Pirin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Pirin Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PIR.

Application Details

Application Notes: PIR antibody can be used for detection of PIR by ELISA at 1:312500. PIR antibody can be used for detection of PIR by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PIR antibody at -20 °C.

Target Details for Pirin

Target: Pirin (PIR)

Alternative Name: PIR

Background: PIR is a member of the cupin superfamily. The protein is an Fe (II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication.This gene encodes a member of the cupin superfamily. The encoded protein is an Fe (II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described.

Molecular Weight: 32 kDa, 32 kDa

Gene ID: 8544

NCBI Accession: NP_003653

UniProt: O00625