CNOT3 antibody

Catalog No. ABIN2460917

The Rabbit Polyclonal anti-CNOT3 antibody has been validated for WB and ELISA. It is suitable to detect CNOT3 in samples from Human, Mouse, Rat and Dog.

Quick Overview for CNOT3 antibody (ABIN2460917)

Target: CNOT3 (CCR4-NOT Transcription Complex, Subunit 3 (CNOT3))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNOT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CNOT3 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CNOT3.

Application Details

Application Notes: CNOT3 antibody can be used for detection of CNOT3 by ELISA at 1:312500. CNOT3 antibody can be used for detection of CNOT3 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store CNOT3 antibody at -20 °C.

Target Details for CNOT3

Target: CNOT3 (CCR4-NOT Transcription Complex, Subunit 3 (CNOT3))

Alternative Name: CNOT3

Background: CNOT3 is a protein component of CCR4-NOT protein complex. Yeast CCR$-NOT is a global regulator of RNA polymerase II transcription. It is comprised of yeast NOT1 to NOT5, yeast CCR4 and additional proteins like yeast CAF1.

Molecular Weight: 82 kDa

Gene ID: 4849

NCBI Accession: NP_055331

UniProt: O75175

Pathways: Stem Cell Maintenance