MIER1 antibody

Catalog No. ABIN2460961

This Rabbit Polyclonal antibody specifically detects MIER1 in WB and ELISA. It exhibits reactivity toward Human, Mouse, Rat, Dog and Zebrafish (Danio rerio).

Quick Overview for MIER1 antibody (ABIN2460961)

Target: MIER1 (Mesoderm Induction Early Response 1 (MIER1))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIER1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MIER1 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MIER1.

Application Details

Application Notes: MIER1 antibody can be used for detection of MIER1 by ELISA at 1:312500. MIER1 antibody can be used for detection of MIER1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MIER1 antibody at -20 °C.

Target Details for MIER1

Target: MIER1 (Mesoderm Induction Early Response 1 (MIER1))

Alternative Name: MIER1

Background: The association of hMI-ER1 with Sp1 represents a novel mechanism for the negative regulation of Sp1 target promoters. Results demonstrate that alternate use of a facultative intron regulates the subcellular localization of hMI-ER1 proteins and this may have important implications for hMI-ER1 function in the regulation of transcription. Repressor activity is due to interaction and recruitment of a trichostatin A-sensitive histone deacetylase 1 (HDAC1).

Molecular Weight: 49 kDa

Gene ID: 57708

NCBI Accession: NP_065999

UniProt: Q8NES5

Pathways: Chromatin Binding