DDX47 antibody

Catalog No. ABIN2461351

This Rabbit Polyclonal antibody specifically detects DDX47 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Dog.

Quick Overview for DDX47 antibody (ABIN2461351)

Target: DDX47 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 47 (DDX47))

Reactivity: Human, Mouse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX47 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DDX47 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDX47.

Application Details

Application Notes: DDX47 antibody can be used for detection of DDX47 by ELISA at 1:1562500. DDX47 antibody can be used for detection of DDX47 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store DDX47 antibody at -20 °C.

Target Details for DDX47

Target: DDX47 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 47 (DDX47))

Alternative Name: DDX47

Background: DDX47 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Molecular Weight: 50 kDa, 45 kDa

Gene ID: 51202

NCBI Accession: NP_057439

UniProt: Q9H0S4