DDX47 antibody (C-Term)

Catalog No. ABIN2776687

This Rabbit Polyclonal antibody specifically detects DDX47 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Rabbit, Horse, Guinea Pig, Cow and Saccharomyces cerevisiae.It has been mentioned in 1 publication

Quick Overview for DDX47 antibody (C-Term) (ABIN2776687)

Target: DDX47 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 47 (DDX47))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Horse, Guinea Pig, Cow, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX47 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-DDX47 Antibody

Binding Specificity: C-Term

Purpose: DDX47 Antibody - C-terminal region

Sequence: AQRFARMELR EHGEKKKRSR EDAGDNDDTE GAIGVRNKVA GGKMKKRKGR

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 86%, Human: 100%, Mouse: 79%, Rabbit: 86%, Rat: 93%, Yeast: 75%

Characteristics: This is a rabbit polyclonal antibody against DDX47. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human DDX47

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8098626

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-DDX47 antibody (ABIN2776687)

Huang, Chien, Huang, Ni, Chang, Ho, Lee, Hwu: "Viral infections and prolonged fever after liver transplantation in young children with inborn errors of metabolism." in: Journal of the Formosan Medical Association = Taiwan yi zhi, Vol. 104, Issue 9, pp. 623-9, (2005) (PubMed).

Target Details for DDX47

Target: DDX47 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 47 (DDX47))

Alternative Name: DDX47

Background:

Background Information: DDX47 encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene can shuttle between the nucleus and the cytoplasm, and has an RNA-independent ATPase activity. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Gene Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 47

Alternative Symbols: RRP3, E4-DBP, HQ0256, MSTP162

Protein Name: Probable ATP-dependent RNA helicase DDX47

Molecular Weight: 50kDa

Gene ID: 51202

NCBI Accession: NP_057439

UniProt: Q9H0S4